Ebrahim Osdaghi, S. Mohsen Taghavi, Mustafa Ojonuba Jibrin, Ralf Koebnik, and Jay Ram Lamichhane (2018)

Multilocus sequence analysis reveals a novel phylogroup of Xanthomonas euvesicatoria pv. perforans causing bacterial spot of tomato in Iran

Plant Pathology : in press

Available at https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12864

A multilocus sequence analysis (MLSA) was performed on five housekeeping genes (i.e. fusA, gapA, gltA, lacF, and lepA) of 22 Xanthomonas euvesicatoria strains recently isolated from alfalfa, pepper and tomato plants in Iran. In addition, 161 strains isolated worldwide from pepper, poinsettia, rose and tomato plants are also included in the analysis. All X. euvesicatoria pv. perforans strains isolated from tomato plants in Iran clustered in a monophyletic group, although five MLSA haplotypes were detected among them. The Iranian tomato strains present 10 nucleotides differences in the lepA gene sequences, compared to the known worldwide population of X. euvesicatoria pv. perforans. Statistical analyses revealed a recombination event that had occurred in the lepA gene of the strains isolated from tomato in Iran. BOX PCR analysis confirmed the inclusion of Iranian tomato strains within X. euvesicatoria pv. perforans. Furthermore, X. euvesicatoria pv. euvesicatoria strains isolated from pepper in Iran differed in one nucleotide in the lepA gene sequence from the known worldwide population of the pathovar, and clustered in a group containing strains isolated in Nigeria. The strains isolated from alfalfa in Iran clustered with the type strain of X. euvesicatoria pv. alfalfae. Altogether, our results reveal the existence of a phylogenetically novel population of X. euvesicatoria pv. perforans in Iran which needs further in-depth analysis to pinpoint the epidemiological impact of these strains.

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